CD24 Antibody Staining Protocol for Immunohistochemistry


Description: CD24 is a 35-45 kDa GPI-linked membrane sialoglycoprotein that is expressed on pro-B, pre-B, and mature B cells, and its expression is decreased after B cell activation. CD24 is also found on granulocytes and a small fraction of thymoctes and neuroblastomas, but not on plasma cells. CD24 may play a role in the regulation of B cell proliferation and differentiation.


Primary Antibody

Name: CD24 (C-20)

Clone: Polyclonal Goat ant-Human

Supplier: Santa Cruz Biotechnology

Catalog Number: sc-7034

Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60 min/room temperature

Antigen Retrieval
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)
Heat/Cool Temperature: 95 ºC - 100 ºC/room temperature
Heat/Cool Time: 20 minutes/20 minuters

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Membrane
Images: Search image

Additional Information:
Species Reactivity: Human, rat
Fixation: Formalin fixed paraffin sections.
Positive Control: Spleen, lymphocytes
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary