Description: CD25 antigen, the alpha subunit of interleukin-2 receptor, is a single-chain glycoprotein with a molecular weight of 55kD. Following the activation of T cells with antigen or mitogen in the presence of the monokine interleukin-1, interleukin 2 (IL-2) is rapidly synthesised and secreted. In response to this, a subpopulation of T cells expresses high affinity receptors for IL-2. These cells proliferate, expanding the T cell population which is capable of mediating helper, suppressor and cytotoxic functions. IL-2 receptor is not uniquely found on T cells and is expressed on HTLV-I transformed T and B cells, EBV transformed B cells, myeloid precursors and oligodendrocytes. It is absent on thymocytes, resting T cells, non-activated B cells and null cells.
Primary Antibody
Name: CD25 Antibody |
Clone: Tu69, Mouse anti-Human |
Supplier: Novocastra Labs |
Catalog Number: NCL-CD25-305 |
Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Membrane |
Images: Search image |
Tissue Type: Tonsil, spleen, thymus |
Fixation: Formalin fixed paraffin sections. Not recommended for acetone fixed frozen sections |
Positive Control: Tonsil, spleen, thymus |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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