Description: The CD4 molecule (T4) is a single chain transmembrane glycoprotein with a molecular weight of 59kD. The CD4 antigen is found on a T cell subset (helper/inducer) representing 45 per cent of peripheral blood lymphocytes. It is also present on 80 per cent of thymocytes and at a lower level on monocytes.
Primary Antibody
Name: CD4 Antibody |
Clone: 1F6, Mouse anti-Human |
Supplier: Novocastra Labs |
Catalog Number: NCL-CD4-1F6 |
Dilution: 1:20 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Membrane (T cells) |
Images: Search image |
Tissue Type: Tonsil |
Fixation: Formalin fixed paraffin sections |
Positive Control: Tonsil, spleen, thymus |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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