Description:
CD68 is a 110 kD transmembrane glycoprotein which is
highly expressed in human monocytes including tissue macrophages and
was identified to be a member of a group of hematopoietic molecules
including CD43, CD34 and the lymph node ligand for L-selectin
GlyCAM-1.1 The antigen is thought to play a role in endocytosis
and/or lysosomal traffic.
Primary Antibody
Name: CD68, Macrophage Antibody |
Clone: KP11, Mouse anti-Human |
Supplier: Dako |
Catalog Number: M0814 |
Dilution: 1:2000 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Skin |
Fixation: Formalin fixed paraffin sections |
Positive Control: All tissue macrophages such as kidney, pancreas, skin, etc. |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
References:
2. Caffo M., et al (2005) CD68 and CR3/43 immunohistochemical expression in secretory meningiomas. Neurosurgery. 57(3):551-7; discussion 551-7. PubMed Abstract
3. Li WV, et al (1996) Immunohistochemical characterization of mast cell disease in paraffin sections using tryptase, CD68, myeloperoxidase, lysozyme, and CD20 antibodies. Mod Pathol. 9(10):982-8. PubMed Abstract