Description: CD71, also known as the transferrin receptor, is a 95 kDa type II membrane glycoprotein that is present as a disulfide-linked homodimer. CD71 binds to two molecules of transferrin, which is a serum iron-transport protein, and it directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is not expressed on resting leukocytes but is upregulated upon activation of lymphocytes, monocytes and macrophages. CD71 is also found on most dividing cells and on brain endothelium.
Primary Antibody
Name: CD71 (Ber-T9) Antibody |
Clone: Mouse monoclonal ant-Human |
Supplier: Santa Cruz Biotechnology |
Catalog Number: sc-19675 |
Dilution: 1:50 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 min/room temperature |
Device: N/A |
Buffer/pH value: N/A |
Heat/Cool Temperature: N/A |
Heat/Cool Time: N/A |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cell membrane |
Images: Search image |
Species Reactivity: Human |
Fixation: Acetone fixed frozen sections |
Positive Control: Human breast carcinoma, skin |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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