CD71 (Ber-T9) Antibody Staining Protocol for Immunohistochemistry

 

Description: CD71, also known as the transferrin receptor, is a 95 kDa type II membrane glycoprotein that is present as a disulfide-linked homodimer. CD71 binds to two molecules of transferrin, which is a serum iron-transport protein, and it directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is not expressed on resting leukocytes but is upregulated upon activation of lymphocytes, monocytes and macrophages. CD71 is also found on most dividing cells and on brain endothelium.

Primary Antibody

Name: CD71 (Ber-T9) Antibody

Clone: Mouse monoclonal ant-Human

Supplier: Santa Cruz Biotechnology

Catalog Number: sc-19675

Dilution: 1:50 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60 min/room temperature

Antigen Retrieval

Device: N/A

Buffer/pH value: N/A

Heat/Cool Temperature: N/A

Heat/Cool Time: N/A

Detection Methods

Standard Method: ABC Method or LSAB Method

Enhanced Method: Polymeric Methods

Chromogen Substrate

Reagent: DAB

Incubation Time/Temperature: 1-3 minutes/room temperature

Counterstain

Reagent: Mayer's Hematoxylin

Staining Time: 30 seconds

Results:

Staining Pattern: Cell membrane

Images: Search image

Additional Information:

Species Reactivity: Human

Fixation: Acetone fixed frozen sections

Positive Control: Human breast carcinoma, skin

Negative Control: Omit primary antibody, isotype control, absorption control

Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary

 

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