CD71 (Ber-T9) Antibody Staining Protocol for Immunohistochemistry


Description: CD71, also known as the transferrin receptor, is a 95 kDa type II membrane glycoprotein that is present as a disulfide-linked homodimer. CD71 binds to two molecules of transferrin, which is a serum iron-transport protein, and it directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is not expressed on resting leukocytes but is upregulated upon activation of lymphocytes, monocytes and macrophages. CD71 is also found on most dividing cells and on brain endothelium.

Primary Antibody

Name: CD71 (Ber-T9) Antibody

Clone: Mouse monoclonal ant-Human

Supplier: Santa Cruz Biotechnology

Catalog Number: sc-19675

Dilution: 1:50 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60 min/room temperature

Antigen Retrieval
Device: N/A
Buffer/pH value: N/A
Heat/Cool Temperature: N/A
Heat/Cool Time: N/A

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Cell membrane
Images: Search image

Additional Information:
Species Reactivity: Human
Fixation: Acetone fixed frozen sections
Positive Control: Human breast carcinoma, skin
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary