Description:
Chromogranin A is an acidic, soluble protein first identified in
adrenal chromoffin granules. Immunohistochemical studies have
demonstrated chromogranin A in most granule-containing
Primary Antibody
Name: Chromogranin A Antibody |
Clone: Polyclonal, Rabbit anti-Human |
Supplier: Dako |
Catalog Number: A0430 |
Dilution: 1:1500 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Stomach, colon, pancreas, adrenal gland, peripheral nerve |
Fixation: Formalin fixed paraffin sections |
Positive Control: Stomach, colon, pancreas, adrenal gland, peripheral nerve |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
1. CGA (Clone LK2H10, Boehringer Mannhein, Cat# 1199021) and (Clone LK2H10+PHE5, Lab Vision, Cat MS-382-P) also works well at 1:5000 with Tris-EDTA pretreatment and 1:3000 with citrate buffer pretreatment, respectively. |
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