Description: The antibody reacts with a large number of epithelial cell types including many ductal and glandular epithelia. In general, it does not react with squamous epithelium. Mixed epithelia of glands consist of two distinct cell types where cytokeratin 8 and 18 are expressed in the luminal cells. Like all cytokeratins, cytokeratin 18 is, in general, not expressed by cells of non-epithelial origin. The antibody does not recognize other intermediate filament proteins.
Primary Antibody
Name: Cytokeratin 18 Antibody |
Clone: DC10, Mouse anti-Human |
Supplier: Dako |
Catalog Number: M7010 |
Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Skin |
Fixation: Formalin fixed paraffin sections, acetone fixed frozen sections, or cytospin preparations. |
Positive Control: Skin, kidney, lung |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
There is no need to perform antigen retrieval for frozen sections. |
References: