Description:
Cytokeratins are intermediate filament cytoskeletal proteins
essential to development and differentiation of epithelial cells.
Approximately twenty different cytokeratins have been identified and
are classified and numbered according to molecular weight and
isoelectric points. In general, most low molecular weight
cytokeratins (40-54 kDa) are distributed in nonsquamous epithelium,
Moll’s catalog numbers 7-8 and/or 17-20. High molecular weight
cytokeratins (48-67 kDa) are found in the squamous epithelium,
Moll’s catalog numbers 1-6 and/or 9-16. Cytokeratin 7 is one of the
basic type II cytokeratins and is found in most glandular epithelia
and in transitional epithelia. The cytokeratin has been identified
as cytokeratin 7 according to the classification of Moll et al.
Monoclonal mouse anti-human Cytokeratin 7, Clone OV-TL 12/30
(anti-CK7) is to identify epithelial cells in normal and neoplastic
tissues using immunohistochemical methods. Anti-CK7 is valuable as a
histodiagnostic tool for the subtyping of various carcinomas and
tumors of epithelial origin. Anti-CK7 may provide a more precise
classification of many epithelial tumors whose differential
diagnosis is otherwise difficult.
Primary Antibody
Name: Cytokeratin 7 Antibody |
Clone: OV-TL 12/30, Mouse anti-Human |
Supplier: Dako |
Catalog Number: M7018 |
Dilution: 1:300 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic/membrane |
Images: Search image |
Tissue Type: Lung, ovary, cervix, endometrium, and breast, skin. |
Fixation: Formalin fixed paraffin sections, acetone fixed frozen sections, or cell smears. |
Positive Control: Lung, ovary, cervix, endometrium, and breast, skin. |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
There is no need to perform antigen retrieval on frozen sections or cell smears |
References: