Cytokeration 7 Antibody Staining Protocol for Immunohistochemistry

Description: Cytokeratins are intermediate filament cytoskeletal proteins essential to development and differentiation of epithelial cells. Approximately twenty different cytokeratins have been identified and are classified and numbered according to molecular weight and isoelectric points. In general, most low molecular weight cytokeratins (40-54 kDa) are distributed in nonsquamous epithelium, Moll’s catalog numbers 7-8 and/or 17-20. High molecular weight cytokeratins (48-67 kDa) are found in the squamous epithelium, Moll’s catalog numbers 1-6 and/or 9-16. Cytokeratin 7 is one of the basic type II cytokeratins and is found in most glandular epithelia and in transitional epithelia. The cytokeratin has been identified as cytokeratin 7 according to the classification of Moll et al. Monoclonal mouse anti-human Cytokeratin 7, Clone OV-TL 12/30 (anti-CK7) is to identify epithelial cells in normal and neoplastic tissues using immunohistochemical methods. Anti-CK7 is valuable as a histodiagnostic tool for the subtyping of various carcinomas and tumors of epithelial origin. Anti-CK7 may provide a more precise classification of many epithelial tumors whose differential diagnosis is otherwise difficult.