Description:
Cytokeratins are a family of
water-insoluble intermediate filaments, composed of polypeptide
chains found in the cytoskeleton of epithelial cells. Twenty
different forms of human cytokeratins exist (Moll numbers 1-20) in
both normal and malignant epithelial cell lines.
Specific
combinations of cytokeratin peptides are associated with certain
epithelial cells, and are therefore useful in the characterization
of poorly differentiated carcinoma. Cytokeratins may also be
clinically significant indicators in distinguishing carcinoma from
malignant tumors of non-epithelial origin, such as lymphoma,
sarcoma, and melanoma. Zymed’s Zym5.2 antibody detects human
cytokeratins with molecular weights 52 and 45 kDa, corresponding to
cytokeratins 8 and 18, respectively. This antibody is useful in the
detection of adenocarcinomas, esotheliomas, and certain squamous
epithelia-derived carcinomas. Interestingly, some epithelial tumors
demonstrate cytokeratin 8 and 18 expression, but normal stratified
squamous epithelium does not. Cytokeratin 8 and 18 staining has also
been observed in renal cell carcinomas, hepatocellular carcinomas,
cholangiocarcinomas, germ cell tumors, and some neuroendocrine
carcinomas, epithelioid sarcomas, and non-epithelial tumors.
Primary Antibody
Name: Cytokeratin 8&18 Antibody |
Clone: UCD/PR-10.11, Mouse monoclonal |
Supplier: Zymed Labs |
Catalog Number: 08-1213 |
Dilution: Pre-dilute |
Incubation Time/Temp: 60 minutes/room temperature |
Device: Steamer |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic/membrane |
Images: Search image |
Tissue Type: Skin, pancreas |
Fixation: Formalin fixed paraffin sections, or acetone fixed frozen sections. |
Positive Control: Skin, pancreas |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
There is no need to perform antigen retrieval for frozen sections. |
References: