Description:
Cytokeratins are a subfamily of intermediate filament proteins and
are characterized by a remarkable biochemical diversity, represented
in epithelial tissues by at least 20 different polypeptides. They
range in molecular weight from between 40 kDa and 68 kDa and
isoelectric pH between 4.9 - 7.8, The individual cytokeratin
polypeptides are designated 1-20. The various epithelia in the human
body usually express cytokeratins, which are not only characteristic
of the type of epithelium, but also related to the degree of
maturation or differentiation within an epithelium. Cytokeratin
subtype expression patterns are used to an increasing extent in the
distinction of different types of epithelial malignancies.
MAB3224 is a broadly reacting cytokeratin antibody which reacts with
cytokeratin 5 among others. It stains basal cells in combined and
stratified epithelial tissues. It recognizes the stem cell
population, including the so-called amplifying cell in the prostate
epithelium.
Primary Antibody
Name: Cytokeratin, Basal Cell Antibody |
Clone: Mouse monoclonal |
Supplier: Chemicon |
Catalog Number: MAB3224 |
Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Stomach |
Fixation: Formalin fixed paraffin sections, or acetone fixed frozen sections |
Positive Control: Stomach |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
There is no need to perform antigen retrieval for frozen sections. |
References: