Description: Cytokeratins are a family of more than 20 structurally related proteins that are differentially expressed in different epithelia and at various stages of differentiation and development. Cytokeratins belong to two families, type I (acidic) and type II (basic), which are usually co-ordinately synthesized in pairs so that at least one member of each family is expressed in each epithelial cell. NCL-C11 reacts with simple epithelium and both basal and suprabasal layers of cornifying and non-cornifying squamous epithelium.
Primary Antibody
Name: Multi-Cytokeratin (4/5/6/8/10/13/18) Antibody |
Clone: C-11, Mouse anti-Human |
Supplier: Novocastra Labs |
Catalog Number: NCL-C11 |
Dilution: 1:20 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Skin |
Fixation: Formalin fixed paraffin sections, or acetone fixed frozen sections. |
Positive Control: Skin |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
There is no need to perform antigen retrieval for frozen sections. |
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