Description: Ciliary neurotrophic factor receptor (CNTFR) shares functional and structural properties with members of the hematopoietic cytokine family. Signaling through the CNTFR involves the binding and activation of a multisubunit receptor complex composed of three components: a ligand-specific-α-receptor (CNTFR), signal-transducing β-subunits of gp130 and leukemia inhibitory factor receptor-β (LIFR).1 Binding of CNTF to CNTFR triggers the association of the receptor complex components resulting in activation of a signal transduction cascade mediated by intracellular protein tyrosine kinases. CNTFR can function in a membrane–bound or soluble form; the membrane-bound form is expressed in neuronal and muscle cells, while the soluble form, once processed, can serve as a cofactor to potentiate CNTF actions. This action may serve to enhance the survival, proliferation, and maturation of oligodendrocyte lineage cells.
Primary Antibody
Name: CNTFR alpha Antibody |
Clone: AN-H6, Mouse monoclonal |
Supplier: BD PharMingen |
Catalog Number: 558783 |
Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Membrane |
Images: Search image |
Tissue Type: Heart |
Fixation: Formalin fixed paraffin sections |
Positive Control: Heart |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
Not tested on frozen sections |
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