Description: Collagen Type IV extracted and purified from mouse tumor tissues. Antibody shows less than 0.1% reactivity with human Collagen types IV, V and mouse Collagen Types I, II, III and mouse Fibronectin. It shows 4.0% reactivity with mouse Laminin by radioimmunoassay.
Primary Antibody
Name: Collagen IV Antibody |
Clone: Rabbit anti-Mouse |
Supplier: Chemicon |
Catalog Number: AB756P |
Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95 ºC - 100 ºC /room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Kidney |
Fixation: Acetone or methyl-carnoy fixed paraffin-embedded sections or frozen sections. |
Positive Control: Kidney |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
No need to perform antigen retrieval on frozen sections |
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