Cox-2 Antibody Staining Protocol for Immunohistochemistry

Description: Prostaglandin G/H synthase (PGHS) catalyzes the first two steps in prostaglandin, thromboxane and prostacyclin synthesis. Two forms of PGHS have now been identified, cloned and characterized. Both forms of PGHS are heme proteins composed of two 70,000 Dalton subunits, and possess both cyclooxygenase and peroxidase activity. Inhibition of the cyclooxygenase activity of PGHS is responsible for the anti-inflammatory activity of aspirin, indomethacin, ibuprofen, piroxicam, and other non-steroidal anti-inflammatory drugs (NSAIDs). The peroxidase activity of PGHS catalyzes oxidation of a broad range of foreign compounds. Whereas PGHS-1 (COX-1) is constitutively expressed in certain cell types, PGHS-1 and PGHS-2 (COX-2)  are induced in response to a number of hormonal and membrane active agents. This antibody was generated against a synthetic peptide corresponding to a distinct C-terminal region of murine PGHS-2. It specifically reacts with PGHS-2 from most species thus far examined. It does not cross-react with PGHS-1.