Description:
Prostaglandins are a diverse group of
autocrine and paracrine hormones that mediate many cellular and
physiologic processes. Prostaglandin H2 (PGH2) is an intermediate in
formation of the prostaglandins. Two prostaglandin synthases that
catalyze the formation of PGH2 from arachidonic acid (AA) are
cyclooxygenase-1 and cyclooxygenase-2. Cyclooxygenase-2, or Cox-2,
is efficiently induced in migratory cells responding to
pro-inflammatory stimuli and is considered to be an important
mediator of inflammation. An alternative form of the protein,
designated Cox-1, is constitutively expressed in most tissues and is
thought to serve in general “housekeeping” functions. Both enzymes
are targets for the nonsteroidal therapeutic anti-inflammatory
drugs, NSAIDs. Cox-2 migrates with an apparent molecular weight of
between 72-74 kDa, while Cox-1 is somewhat smaller, migrating at 70
kDa.
Primary Antibody
Name: Cox-2 (M-19) Antibody |
Clone: Goat polyclonal |
Supplier: Santa Cruz Biotechnology |
Catalog Number: sc-1747 |
Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95 ºC - 100 ºC /room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Membrane/cytoplasmic |
Images: Search image |
Tissue Type: Colon, kidney |
Fixation: Formalin fixed paraffin sections |
Positive Control: Colon, kidney |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
Not tested on frozen sections |
References: