Description: CRH is a 41-amino acid peptide derived by enzymatic cleavage from a 191-amino acid preprohormone and is located on 8q13. It is made not only in the hypothalamus but also in peripheral tissues, such as T lymphocytes, and is expressed in very large amounts in the human placenta. As a placental clock, CRH controls the duration of pregnancy and that the timing of the rise in CRH may permit prediction of the onset of labor. Furthermore, CRH is the most proximal element of the HPA axis, and it acts as a central coordinator for neuroendocrine and behavioral responses to stress. In addition, placental secretion of CRH is a marker of the 'placental clock' that is active from an early stage in human pregnancy and determines the length of gestation and the timing of parturition and delivery.
Primary Antibody
Name: CRH IHC Antibody |
Clone: Rabbit Polyclonal |
Supplier: IHC World |
Catalog Number: IW-PA1128 |
Dilution: Ready to Use |
Incubation Time/Temp: 60 min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Secreted |
Images: Search image |
Species Reactivity: Human, mouse, rat |
Fixation: Formalin fixed paraffin embedded sections or acetone fixed frozen sections. |
Positive Control: Brain (hypothalamus), placenta, thymus. |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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