Dendritic Cells Antibody Staining Protocol for Immunohistochemistry


Description: Specificity: Anti-Mouse Dendritic (clone 33D1) recognizes a mouse cell surface antigen on dendritic cells. Antigen Distribution: The antibody is cytotoxic and does not appear to be directed against la or immune response associated antigen. The antigen is present only on dendritic cells with an estimated frequency of 10,000-15,000 sites per cell.


Primary Antibody

Name: Dendritic Cells Antibody

Clone: 33D1, Rat anti-Mouse

Supplier: Leino Technologies

Catalog Number: D104

Dilution: 1:500 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60 minutes/room temperature

Antigen Retrieval
Device: N/A
Buffer/pH value: N/A
Heat/Cool Temperature: N/A
Heat/Cool Time: N/A

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Membrane
Images: Search image

Additional Information:
Tissue Type: Mouse spleen
Fixation:  Acetone or formalin fixed frozen sections
Positive Control: Mouse spleen
Negative Control: Omit primary antibody, isotype control or absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary


Not tested on paraffin sections