Description: Desmin is a 53 kDa intermediate filament protein present in smooth muscle cells, striated muscle cells and myocardium. In skeletal and cardiac muscle cells, desmin filaments are localized in the Z-bands.
Primary Antibody
Name: Desmin Antibody |
Clone: DE-R-11, Mouse anti-Porcine |
Supplier: Dako |
Catalog Number: M0724 |
Dilution: 1:30 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95 ºC - 100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Tissue Type: Skeletal and cardiac muscle |
Fixation: Formalin fixed paraffin sections, or acetone fixed frozen sections |
Positive Control: Skeletal and cardiac muscle cells, smooth muscle cells of blood vessels, viscera, the uterus as well as in Purkinje cells, many mesangial cells of the kidney glomeruli, leiomyoma and rhabdomyomas. |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
No need to perform antigen retrieval on frozen sections |
References: