Description: Elastin is a polymeric protein found in connective tissue that imparts the property of elasticity to vertebrate elastic tissue. It is synthesized and secreted as a soluble, single-chain protein (tropoelastin) which undergoes a number of post-ribosomal modifications prior to its organization into an elastic fiber in the extracellular space. Once secreted, tropoelastin molecules are joined covalently via chemical modification and cross-linking of specific lysyl residues to form the mature insoluble elastin. Ultrastructurally, it is predominantly an amorphous material which may change its morphology with ageing and different disease states. The abnormal accumulation of elastic tissue in blood vessels is found in atherosclerosis and hypertension. Genetic defects in the elastin molecule may result in heritable diseases such as Marfan's syndrome, pseudoxanthoma elasticum and the Bushke-Ollendorf syndrome.
Primary Antibody
Name: Elastin Antibody |
Clone: BA-4, Mouse anti-Human |
Supplier: Novocastra |
Catalog Number: NCL-Elastin |
Dilution: 1:200 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: Incubator |
Buffer/pH value: IHC-TekTM Proteinase K Solution (Cat# IW-1101) |
Heat/Cool Temperature: 37 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Extracellular elastic fibers |
Images: Search image |
Tissue Type: Kidney, skin, small intestine, liver. |
Fixation: Formalin fixed paraffin sections |
Positive Control: Kidney, skin, small intestine, liver. |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
This antibody does not work on acetone fixed frozen sections. |
References: