Description: Anti-EMA, E29 reacts with a variety of normal epithelial tissues including: secretory and ductal epithelial cells of the skin, breast and other organs of glandular differentiation including sebaceous, esophageal and gastric glands.
Primary Antibody
Name: EMA Antibody |
Clone: E29, Mouse anti-Human |
Supplier: Dako |
Catalog Number: M0613 |
Dilution: 1:500 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95 ºC - 100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic and plasma membrane |
Images: Search image |
Tissue Type: Skin, Breast cancer, Colon cancer |
Fixation: Formalin fixed paraffin sections, or acetone fixed frozen sections, or cell smears |
Positive Control: Skin, Breast cancer, Colon cancer |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
No need to perform antigen retrieval on frozen sections |
References: