Description: Epithelial membrane antigen (EMA), is a mucin-like glycoprotein. Antibody to EMA has been shown useful as a pan- epithelial marker for detecting early metastatic loci of carcinoma in the bone marrow or liver.
Primary Antibody
Name: EMA Ab-3 Antibody |
Clone: E29, Mouse anti-Human |
Supplier: Lab Vision |
Catalog Number: MS-741 |
Dilution: 1:50 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: No antigen retrieval is needed. |
Buffer/pH value: N/A |
Heat/Cool Temperature: N/A |
Heat/Cool Time: N/A |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic and cell membrane |
Images: Search image |
Tissue Type: Breast cancer, Colon carcinoma |
Fixation: Formalin fixed paraffin sections |
Positive Control: Breast cancer, Colon carcinoma |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
Not tested on frozen sections |
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