Description: The CI: A3-1 monoclonal antibody (mAb) reacts with the F4/80 antigen which is a macrophage - restricted cell surface glycoprotein. Applications of the CI: A3-1 mAb include immunohistochemistry and immunostaining for flow cytometry.
Primary Antibody
Name: F4/80 Antibody |
Clone: CI:A3-1, Rat anti-Mouse |
Supplier: Caltag Labs (Invitrogen) |
Catalog Number: RM290 |
Dilution: 1:50 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: Incubator |
Buffer/pH value: IHC-TekTM Proteinase K Solution (Cat# IW-1101) |
Heat/Cool Temperature: 37 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Membrane/cytoplasmic |
Images: Search image |
Species Reactivity: Mouse |
Fixation: Formalin fixed paraffin sections |
Positive Control: Spleen |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
Not tested on frozen sections |
References: