Description: Fibronectin is a glycoprotein that has functions associated with cell adhesion and migration. It is found primarily in basement membranes and is a key extracellular matrix component. This antibody will help detect fibronectin in normal and uncharacterized tissues.
Primary Antibody
Name: Fibronectin Antibody |
Clone: 568, Mouse anti-Human |
Supplier: Vector Labs |
Catalog Number: VP-F705 |
Dilution: 1:100 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 minutes/room temperature |
Device: Incubator |
Buffer/pH value: IHC-TekTM Proteinase K Solution (Cat# IW-1101) |
Heat/Cool Temperature: 37 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Extracellular connective tissue matrix, basement membranes |
Images: Search image |
Tissue Type: Skin, kidney |
Fixation: Formalin fixed paraffin sections, or acetone fixed frozen sections, or cell smears. |
Positive Control: Normal kidey (extracellular labeling of connective tissue matrix), basement membrane of skin, blood vessels, smooth muscles, ganglia and muscle fibers. |
Negative Control: Omit primary antibody, isotype control or absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
No need to perform antigen retrieval on frozen sections |
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