Description: Irmler et al. (1997) isolated human cDNAs encoding 2 isoforms of FLIP. FLIP(L), the longer isoform, contains 2 DEDs and a caspase-like domain, and FLIP(S), the shorter isoform, contains only the 2 DEDs followed by a C-terminal extension of approximately 50 amino acids. High levels of FLIP (L) protein were detected in melanoma cell lines and malignant melanoma tumors. FLIP may be implicated in tissue homeostasis as an important regulator of apoptosis.
Primary Antibody
Name: FLIP-L IHC Antibody |
Clone: Rabbit polyclonal |
Supplier: IHC World |
Catalog Number: IW-PA1034 |
Dilution: Ready to Use |
Incubation Time/Temp: 60 min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Species Reactivity: Human, mouse, rat |
Fixation: Formalin fixed paraffin sections |
Positive Control: Human Mammary cancer |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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