FLIP-L Antibody Staining Protocol for Immunohistochemistry

 

Description: Irmler et al. (1997) isolated human cDNAs encoding 2 isoforms of FLIP. FLIP(L), the longer isoform, contains 2 DEDs and a caspase-like domain, and FLIP(S), the shorter isoform, contains only the 2 DEDs followed by a C-terminal extension of approximately 50 amino acids. High levels of FLIP (L) protein were detected in melanoma cell lines and malignant melanoma tumors. FLIP may be implicated in tissue homeostasis as an important regulator of apoptosis.

 

Primary Antibody

Name: FLIP-L IHC Antibody

Clone: Rabbit polyclonal

Supplier: IHC World

Catalog Number: IW-PA1034

Dilution: Ready to Use

Incubation Time/Temp: 60 min/room temperature

Antigen Retrieval

Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)

Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)

Heat/Cool Temperature: 95-100 ºC/room temperature

Heat/Cool Time: 20 minutes/20 minutes

Detection Methods

Standard Method: ABC Method or LSAB Method

Enhanced Method: Polymeric Methods

Chromogen Substrate

Reagent: DAB

Incubation Time/Temperature: 1-3 minutes/room temperature

Counterstain

Reagent: Mayer's Hematoxylin

Staining Time: 30 seconds

Results:

Staining Pattern: Cytoplasmic

Images: Search image

Additional Information:

Species Reactivity: Human, mouse, rat

Fixation: Formalin fixed paraffin sections

Positive Control: Human Mammary cancer

Negative Control: Omit primary antibody, isotype control, absorption control

Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary

 

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