GFAP Antibody Staining Protocol for Immunohistochemistry

 

Description: Glial fibrillary acidic protein (GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.

 

Primary Antibody

Name: GFAP IHC Antibody

Clone: Mouse Monoclonal: GA-8

Supplier: IHC World

Catalog Number: IW-MA1045

Dilution: Ready to Use

Incubation Time/Temp: 60 min/room temperature


Antigen Retrieval
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)
Heat/Cool Temperature: 95-100 ºC/room temperature
Heat/Cool Time: 20 minutes/20 minutes

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Counterstain
Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Results:
Staining Pattern: Cytoplasmic
Images: Search image

Additional Information:
Species Reactivity: Human, mouse, rat
Fixation: Formalin fixed paraffin embedded sections or acetone fixed frozen sections.
Positive Control: Brain
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary

 

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