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Lactoferrin Antibody Staining Protocol for Immunohistochemistry
Description: Lactoferrin is a glycoprotein that belongs to the iron transporter or transferrin family. It was originally isolated from bovine milk and contains 703 amino acids and has a molecular weight of 80 kD. Lactoferrin is considered a multifunctional or multi-tasking protein and appears to play several biological roles. Owing to its iron-binding properties, lactoferrin plays a role in iron uptake by the intestinal mucosa of the suckling neonate. It also has antibacterial, antiviral, antifungal, anti-inflammatory, antioxidant and immunomodulatory activities. The possible antibacterial activity of supplemental lactoferrin might be accounted for, in part, by its ability to strongly bind iron. Iron is essential to support the growth of pathogenic bacteria. Lactoferrin may also inhibit the attachment of bacteria to the intestinal wall.
Primary Antibody
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Name: Lactoferrin Antibody |
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Clone: Mouse monoclonal |
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Supplier: BioDesign |
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Catalog Number: H86024M |
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Dilution: 1:20,000 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
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Incubation Time/Temp: 60 min/room temperature |
Antigen Retrieval
| Device: Antigen retrieval is not needed. |
| Buffer/pH value: N/A |
| Heat/Cool Temperature: N/A |
| Heat/Cool Time: N/A |
Detection Methods
| Standard Method: ABC Method or LSAB Method |
| Enhanced Method: Polymeric Methods |
Chromogen Substrate
| Reagent: DAB |
| Incubation Time/Temperature: 1-3 minutes/room temperature |
Counterstain
| Reagent: Mayer's Hematoxylin |
| Staining Time: 30 seconds |
Results:
| Staining Pattern: Cytoplasmic |
| Images: Search image |
Additional Information:
| Tissue Type: Lactoferrin treated intestine and colon |
| Fixation: Formalin fixed paraffin sections |
| Positive Control: Lactoferrin treated intestine and colon |
| Negative Control: Omit primary antibody, isotype control, absorption control |
| Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
References: