Lactoferrin Antibody Staining Protocol for Immunohistochemistry


Description: Lactoferrin is a glycoprotein that belongs to the iron transporter or transferrin family. It was originally isolated from bovine milk and contains 703 amino acids and has a molecular weight of 80 kD. Lactoferrin is considered a multifunctional or multi-tasking protein and appears to play several biological roles. Owing to its iron-binding properties, lactoferrin plays a role in iron uptake by the intestinal mucosa of the suckling neonate. It also has antibacterial, antiviral, antifungal, anti-inflammatory, antioxidant and immunomodulatory activities. The possible antibacterial activity of supplemental lactoferrin might be accounted for, in part, by its ability to strongly bind iron. Iron is essential to support the growth of pathogenic bacteria. Lactoferrin may also inhibit the attachment of bacteria to the intestinal wall.


Primary Antibody

Name: Lactoferrin Antibody

Clone: Mouse monoclonal

Supplier: BioDesign

Catalog Number: H86024M

Dilution: 1:20,000 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60 min/room temperature

Antigen Retrieval
Device: Antigen retrieval is not needed.
Buffer/pH value: N/A
Heat/Cool Temperature: N/A
Heat/Cool Time: N/A

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Cytoplasmic
Images: Search image

Additional Information:
Tissue Type: Lactoferrin treated intestine and colon
Fixation: Formalin fixed paraffin sections
Positive Control: Lactoferrin treated intestine and colon
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary