Laminin Antibody Staining Protocol for Immunohistochemistry

 

Description: Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1,beta-1 and gamma-1, formerly called beta-2 (LAMA2). This gene is over 260, 000 base pairs and contains 64 exons.  Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins.  And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder.

 

Primary Antibody

Name: Laminin IHC Antibody

Clone: Mouse Monoclonal: LAM-26

Supplier: IHC World

Catalog Number: IW-MA1054

Dilution: Ready to Use

Incubation Time/Temp: 60 min/room temperature

Antigen Retrieval

Device: Incubator

Buffer/pH value: IHC-TekTM Proteinase K (Cat# IW-1101)

Heat/Cool Temperature: 37 ºC/room temperature

Heat/Cool Time: 20 minutes/20 minutes

Detection Methods

Standard Method: ABC Method or LSAB Method

Enhanced Method: Polymeric Methods

Chromogen Substrate

Reagent: DAB

Incubation Time/Temperature: 1-3 minutes/room temperature

Counterstain

Reagent: Mayer's Hematoxylin

Staining Time: 30 seconds

Results:

Staining Pattern: Basement membrane

Images: Search image

Additional Information:

Species Reactivity: Human, pig, feline

Fixation: Formalin fixed paraffin embedded sections or acetone fixed frozen sections.

Positive Control: Skin, kidney, skeletal muscle, small intestine.

Negative Control: Omit primary antibody, isotype control, absorption control

Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary

 

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