Description: Laminin is a heterotrimeric extracellular matrix protein consisting of 3 chains: alpha-1,beta-1 and gamma-1, formerly called beta-2 (LAMA2). This gene is over 260, 000 base pairs and contains 64 exons. Laminin is similar with merosin, a basement membrane-associated protein found in placenta, striated muscle, and peripheral nerve, and both of them are members of the same family of basement membrane proteins. And merosin is the same as laminin M, a striated muscle-specific, basal-lamina-associated protein, it may play a primary role in the pathogenesis of that disorder.
Primary Antibody
Name: Laminin IHC Antibody |
Clone: Mouse Monoclonal: LAM-26 |
Supplier: IHC World |
Catalog Number: IW-MA1054 |
Dilution: Ready to Use |
Incubation Time/Temp: 60 min/room temperature |
Device: Incubator |
Buffer/pH value: IHC-TekTM Proteinase K (Cat# IW-1101) |
Heat/Cool Temperature: 37 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Basement membrane |
Images: Search image |
Species Reactivity: Human, pig, feline |
Fixation: Formalin fixed paraffin embedded sections or acetone fixed frozen sections. |
Positive Control: Skin, kidney, skeletal muscle, small intestine. |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: Normal serum blocking is not needed for this RTU antibody; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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