Myc Antibody Staining Protocol for Immunohistochemistry


Description: The myc gene family encodes nuclear phosphoproteins that are thought to play a role in the control of cellular proliferation and differentiation. Comparison with Ki-67 staining indicates that myc protein expression in many tumors is uncoupled from cellular proliferation. This antibody's strongest reactivity observed with human and mouse Myc. Will also cross-react with avian and Xenopus.


Primary Antibody

Name: Myc Antibody

Clone: Rabbit anti-Human polyclonal

Supplier: Upstate

Catalog Number: 06-340

Dilution: 1:1000 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed.

Incubation Time/Temp: 60 min/room temperature

Antigen Retrieval
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102)
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100)
Heat/Cool Temperature: 95-100 ºC/room temperature
Heat/Cool Time: 20 minutes/20 minutes

Detection Methods
Standard Method: ABC Method or LSAB Method
Enhanced Method: Polymeric Methods

Chromogen Substrate
Reagent: DAB
Incubation Time/Temperature: 1-3 minutes/room temperature

Reagent: Mayer's Hematoxylin
Staining Time: 30 seconds

Staining Pattern: Nuclear
Images: Search image

Additional Information:
Tissue Type: Colon
Fixation: Formalin fixed paraffin sections
Positive Control: Normal colonic mucosa, colorectal polyps, and colorectal adenocarcinomas, breast carcinoma.
Negative Control: Omit primary antibody, isotype control, absorption control
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary