Description: Somatostatin is one of seven known polypeptide hormones produced in the pancreas. Somatostatin functions as an inhibitory hormone of the neuroendocrine system and is secreted by D-cells of the islets of Langerhans, the fundus and antrum of the stomach and in the upper small intestine. Somatostatin has been demonstrated in sympathetic nerves, mucosal cells, myenteric nerves of the gastrointestinal tract, salivary glands and in some parafollicular cells of the thyroid. This antibody labels D cells of mammalian pancreas. Cross-reactivity has been observed with somatostatin from a large number of mammalian species.
Primary Antibody
Name: Somatostatin Antibody |
Clone: Rabbit anti-Human |
Supplier: Dako |
Catalog Number: A0566 |
Dilution: 1:500 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic |
Images: Search image |
Species Reactivity: Human, mouse, rat |
Fixation: Formalin fixed paraffin sections |
Positive Control: Pancreas (islet cells) |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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