Description: Tyrosine hydroxylase is the first enzyme in catecholamine (CA) biosynthesis and catalyses the conversion of L-tyrosine to L-DOPA. Tyrosine hydroxylase is , therefore, a useful marker of all CA neurons and allows their localization in different areas of the brain.
Primary Antibody
Name: Tyrosine Hydroxylase (TH) Antibody |
Clone: Rabbit polyclonal |
Supplier: Millipore |
Catalog Number: AB152 |
Dilution: 1:100 - 1:500 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB Chromogen Substrate |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Cytoplasmic of dopamine neurons |
Images: Search image |
Species Reactivity: Human, mouse, rat, ferret, feline, Aplysia (marine mollusc). |
Fixation: Formalin fixed paraffin sections or formalin fixed frozen sections |
Positive Control: Brain (striatum, substantia nigra) dopamine neurons |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
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