Description: Thymine dimers are the most dominant form of chemical modification that occur on DNA after exposure to UV-light. It detects thymine dimers in single-stranded DNA, and has a lower affinity for the dimer in short oligonucleotides and is well suited for anyone working in the field of DNA damage and repair.
Primary Antibody
Name: Thymine Dimer Antibody |
Clone: Mouse monoclonal |
Supplier: Affitech |
Catalog Number: 1000-1 |
Dilution: 1:2000 using IHC-TekTM Antibody Diluent (Cat# IW-1000 or IW-1001) to reduce background and unspecific staining and serum blocking step is NOT needed. |
Incubation Time/Temp: 60 min/room temperature |
Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
Heat/Cool Temperature: 95-100 ºC/room temperature |
Heat/Cool Time: 20 minutes/20 minutes |
Standard Method: ABC Method or LSAB Method |
Enhanced Method: Polymeric Methods |
Reagent: DAB |
Incubation Time/Temperature: 1-3 minutes/room temperature |
Reagent: Mayer's Hematoxylin |
Staining Time: 30 seconds |
Staining Pattern: Nuclear |
Images: Search image |
Species Reactivity: Human, mouse, Swine |
Fixation: Formalin fixed paraffin sections. Not suitable for frozen sections. |
Positive Control: Skin (UV exposed) |
Negative Control: Omit primary antibody, isotype control, absorption control |
Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
References: