 |
|
TNF-alpha Antibody Staining Protocol for Immunohistochemistry
Description:
TNFα
(Tumor Necrosis Factor alpha) gene encodes a multifunctional
proinflammatory cytokine that belongs to the tumor necrosis factor
(TNF) superfamily. This cytokine is mainly secreted by macrophages.
It can bind to, and thus functions through its receptors
TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the
regulation of a wide spectrum of biological processes including cell
proliferation, differentiation, apoptosis, lipid metabolism, and
coagulation. This cytokine has been implicated in a variety of
diseases, including autoimmune diseases, insulin resistance, and
cancer. Knockout studies in mice also suggested the neuroprotective
function of this cytokine.
Primary Antibody
|
Name: TNF-alpha IHC Antibody |
|
Clone: Rabbit polyclonal |
|
Supplier: IHC World |
|
Catalog Number: IW-PA1079 |
|
Dilution: Ready to Use |
|
Incubation Time/Temp: 60 min/room temperature |
Antigen Retrieval
| Device: IHC-TekTM Epitope Retrieval Steamer Set (Cat# IW-1102) |
| Buffer/pH value: IHC-TekTM Epitope Retrieval Solution (Cat# IW-1100) |
| Heat/Cool Temperature: 95-100 ºC/room temperature |
| Heat/Cool Time: 20 minutes/20 minutes |
Detection Methods
| Standard Method: ABC Method or LSAB Method |
| Enhanced Method: Polymeric Methods |
Chromogen Substrate
| Reagent: DAB |
| Incubation Time/Temperature: 1-3 minutes/room temperature |
Counterstain
| Reagent: Mayer's Hematoxylin |
| Staining Time: 30 seconds |
Results:
| Staining Pattern: Cytoplasmic |
| Images: Search image |
Additional Information:
| Species Reactivity: Human, mouse, rat |
| Fixation: Formalin fixed paraffin sections |
| Positive Control: Human mammary cancer |
| Negative Control: Omit primary antibody, isotype control, absorption control |
| Blocking: 2-5% normal serum to reduce unspecific background staining; 0.5-3% H2O2 to block endogenous peroxidase activity; avidin/biotin to block endogenous biotin activity if necessary |
References: