Nuclear Fast Red Counterstain Protocol




Nuclear Fast Red (Kernechtrot) Solution


       Nuclear fast red ------------------------- 0.1 g

       Aluminum sulfat ------------------------ 5 g

       Distilled water ---------------------------100 ml

       Dissolve aluminum sulfate (Sigma, Cat# 368458-500G) in water. Add nuclear fast red and slowly heat to boil and cool. Filter and add a grain of thymol as a preservative.


Staining Pattern



Suggested Use

Counterstaining for immunohistochemistry or other special staining.


Suggested Staining Time

5 minutes