Immunocytochemistry Methods, Techniques and Protocols


ICC Main Page > Observation

  1. Examine the cells under the microscope.

  2. Record the results and taking pictures of the labeled cells.


Note: It is advisable to run the appropriate negative controls. Negative controls establish background fluorescence and non-specific staining of the primary and secondary antibodies. The ideal negative control reagent is a fluorochrome conjugated mouse monoclonal or myeloma protein. It should be isotype-matched, not specific for cells of the species being studied and of the same concentration as the test antibody. The degree of autofluorescence or negative control reagent fluorescence will vary with the type of cells under study and the sensitivity of the instrument used. For fluorescent analysis of cells with Fc receptors, the use of isotype-matched negative controls is mandatory.