Aqueous Mounting Medium Protocols

 

 

 

Aqueous Mounting Medium (glycerol gelatin)

 

	Kisser’s	Kaiser's	Glycerol jelly	Modified
Gelatine	10 g	40 g	65 g	5 g
Distilled water	35 ml	210 ml	300 ml	50 ml
Glycerol (glycerin)	30 ml	250 ml	100 ml	50 ml
Phenol (carbolic acid)		5 ml	5 ml

                                                    

 

Mix gelatin and distilled water. Heat in 50 C oven until gelatin is dissolved (it takes about 30 minutes). Add glycerin and adjust pH to 7.0 using 1N NaOH (to avoid fading of hematoxylin). Store at room temperature.

 

Warm to 37-40 C before mounting. Note: Modified protocol works well.

 

See also Making and Using Aqueous Mounting Media