AP Buffer
AP Buffer (100mM Tris-HCl, 100mM NaCl, 5mM MgCl2, 0.05% Tween 20, pH 9.5):
Tris-HCl (MW 157.60) --------------------- 15.8 g
NaCl (MW 58.44) --------------------------- 5.8 g
MgCl2 (MW 203.31) ------------------------ 1.02 g
Distilled water ----------------------------- 900 ml
Mix to dissolve. Adjust pH to 9.5 and
then bring total volume to 1 liter.
Add 0.5 ml Tween 20.
Store this solution at room temperature.