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Suggestions for Control Section Material
Roy C. Ellis
IMVS Division of Pathology
The Queen Elizabeth Hospital
Woodville Road, Woodville, South Australia 5011
Email: roy.ellis@imvs.sa.gov.au
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Acetylcholinesterase |
Cerebellum |
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Acid phosphatase |
Prostate, liver or kidney |
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Adenosine triphosphatase |
Muscle or liver |
| Adrenaline | Adrenal medulla |
| Alkaline phosphatase | Kidney or small intestine |
| Alphafoetoprotein | Primary carcinoma of liver |
| Aluminium | Lung or skin in diseased patient |
| Amyloid AA |
Widespread but kidney, liver, spleen, lymph nodes, adrenal and thyroid are classically involved. |
| Amyloid AL |
Widespread but heart, gastro-intestinal tract, peripheral nerves, skin and tongue are classically involved. |
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Argentaffin cells |
Appendix or ileum |
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Arginine |
Paneth cells and lymphoid tissue |
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Argyrophil cells |
Stomach, small intestine |
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Arsenic |
Hair, nails and bone |
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Ascorbic acid |
Liver or adrenal cortex |
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Aspergillus |
Lung in diseased patient |
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Astrocytes, fibrous |
Brain white matter |
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Astrocytes, protoplasmic |
Brain grey matter |
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Axons |
Cerebrum, cerebellum, spinal cord |
| Basement membranes |
Kidney |
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Beryllium |
Lung in diseased patient |
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Bilirubin |
Biliary cirrhosis, cholelithiasis |
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Biliverdin |
Biliary cirrhosis, cholelithiasis |
| Calcitonin |
Dog thyroid |
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Calcium carbonate |
Bone, necrosis, atheroma |
| Calcium oxalate |
Kidney or thyroid in oxalosis, bone |
| Calcium phosphate |
Bone, necrosis, atheroma |
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Candida albicans |
Skin of diseased patient |
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Carcino-embryonic antigen |
Colon |
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Carcinoid |
Appendix, small intestine, rectum and sigmoid colon. |
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Cartilage, fibrous |
Invertebral discs |
| Cartilage, hyaline |
Joints |
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Charcot-leyden crystals |
Lung of asthmatics, eosinophil granulomas |
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Chitin |
Hydatid cysts of liver and lung |
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Cholesterol |
Adrenal cortex and atheroma |
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Chromaffin |
Adrenal medulla |
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Collagen |
Skin, lung or liver |
| Copper |
Liver in diseased patient - Wilson's disease, primary biliary cirrhosis |
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Cryptococcus |
Lung of diseased patient |
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Cysteine |
Hair follicles |
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Cystine |
Stratum corneum and hair shafts |
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Cytochrome oxidase |
Liver, muscle and kidney |
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Degenerate myelin |
Brain of patients with multiple schlerosis |
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Dehydrogenase |
Liver, kidney and heart |
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Dendrites |
Cerebrum, cerebellum, spinal cord |
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DNA |
Lymph node |
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Dopamine |
Mast cells of mice and rats |
| Elastic fibres |
Aorta, dermis of skin, lung or intestine |
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Epidermis, intercellular bridges |
Squamous cell papilloma, palmar and plantar skin |
| Esterase (non specific) |
Liver, kidney or small intestine |
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Fatty acids |
Fat necrosis |
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Fibrin |
Foetal lung in hyaline membrane disease |
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Ganglion cells |
Walls of the gastro-intestinal tract |
| Glucose-6-phosphatase |
Colon |
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Glycogen |
Liver |
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Golgi apparatus |
Neurones |
| Gram negative organisms |
Controls can be manufactured using a culture from a known organism and lung from autopsy. Culture together for 24 hours then fix and process. |
| Gram positive organisms |
As for gram negative organisms |
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Haematoidin |
Infarcts and abscesses |
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Haemazoin |
Liver of malaria patients |
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Haemosiderin |
Haemorrhage and haemochromotosis |
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Hepatitis B surface antigen |
Liver in viral hepatitis |
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Human chorionic |
Placenta gonadotrophin |
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Hyaluronic acid |
Umbilical cord |
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Iron |
Liver in hemochromatosis |
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Keratin |
Palmar and plantar skin |
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Legionella pneumophilia |
Lung of diseased patient |
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Leprosy bacilli (myco leprae) |
Skin of diseased patient |
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Lipid |
Corpus luteum of ovary |
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Lipofuschin |
Heart, ganglion cells |
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Mallory bodies |
Liver from alcoholic cirrhosis |
| Mast cells |
Gastro-intestinal tract, intermuscular tissue or skin |
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Melanin |
Negroid skin, naevus tumours |
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Microglia |
Brain |
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Mitochondria |
Renal tubules, liver and heart |
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Mucin |
Small intestine, submaxillary gland |
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Mucopolysaccharides |
Skin, umbilical cord |
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Muscle, striations |
Skeletal muscle |
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Myelin |
Cerebrum, cerebellum or spinal cord |
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Neurosecretory substance |
Hypothalmus |
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Neutral fat |
Subcutaneous tissue |
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Neutral mucin |
Stomach |
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Nissl |
Brain or spinal cord |
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Noradrenaline |
Adrenal medulla |
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6-nucleotidase |
Thyroid or liver |
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Osteoid |
Bone in osteomalacia |
| Oxytalan fibres |
Periodontal ligaments |
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Paneth cells |
Small intestine |
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Peroxidase |
Granulocytes, erythrocytes |
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Phospholipid |
Brain or spinal cord |
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Plasma cells |
Rheumatoid synovitis, lymph nodes in plasmacytosis |
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Pneumocystis carinii |
Lung in diseased patient |
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Purkinje cells |
Cerebellum |
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Purkinje fibres |
Heart (subendocardium) |
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Reticulin fibres |
Liver, spleen, lymphoid tissue |
| Ribonucleic acid |
Plasma cell, neurones |
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Rickettsiae |
Brain of diseased patient |
| Russell bodies |
Rheumatoid synovitis |
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Seratonin |
Small intestine (argentaffin cells) |
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Sialomucin (sialidase labile) |
Salivary gland |
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Sialomucin (sialidase resistant) |
Colon |
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Spirochaetes |
Lyophilised T. Pallidum reconstituted, smeared on to clean glass slide and air dried. |
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Steroids |
Adrenal cortex, seminiferous tubules |
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Sulphated mucin |
Cartilage, colon |
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Tryptophan |
Paneth cells, pancreas |
| Tubercle bacilli |
Tissue from diseased patient, commonly lung |
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Tyrosinase |
Skin (melanocytes) |
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Tyrosine |
Pancreas |
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Urates |
Gouty tophi |
| Viral inclusion body |
Surface lesions of diseased patient (herpes). Lung of diseased patient (cytomegalovirus) |
Reference
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Bancroft JD, Stevens HC. Manual of Histologic Techniques. Churchill Livingstone 1984
Fischer RS, Petty CS. Forensic Pathology U.S. Department of Justice, Washington D.C. 1977
Robbins SL, Angell M, Kumar V. Basic Pathology, W.B. Saunders. 1981
Tedeschi CG, Eckert WG, Tedeschi LG. Forensic Medicine. W.B. Saunders. 1977
Wheater PR, Burkitt Hg, Daniels VG. Functional Histology: A text and colour atlas. Churchill Livingstone. 1987
Churukian CJ. Manual of the special stains laboratory. The Department of Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, New York. 1993