Antigen Retrieval Protocols

The demonstration of many antigens can be significantly improved by the pretreatment with the antigen retrieval reagents that break the protein cross-links formed by formalin fixation and thereby uncover hidden antigenic sites.

Antigen Retrieval Overview

• Overview of Heat-induced Epitope Retrieval Techniques and Devices

• Citrate Buffer Method (pH 6)

• Citrate-EDTA Buffer Method (pH 6.2)

• EDTA Method (pH 8)

• Tris-EDTA Method (pH 9)

• TBS Method (pH 9)

• Tris Buffer Method (pH 10)