General IHC Protocols

• Blocking of Unwanted Non-specific Staining

• Immunoenzyme (HRP) Method

• Immunoenzyme (AP) Method

• Immunofluorescence  Method

• Standard ABC (Avidin-Biotin Complex) Method

• Standard LSAB (Labeled StreptAvidin Biotin) Method

• Labeled Polymeric Methods

• Polymer Enhanced Method - A Highly Sensitive IHC Detection Protocol

• CSA (Catalyzed Signal Amplification) Method

• Immunohistochemical Staining Method for Mouse Antibody on Mouse Tissue

• Isotype-specific Immunostaining with Mouse Primary Antibodies on Mouse Tissue

• Immunohistochemistry Protocol on Mouse Spine

• Double Immunohistochemistry Staining Protocol

• Double Immunofluorescence Staining for Bcl-6 and else

• Immunohistochemistry Double Staining Method

• Immunoflurescence Double Staining Method - Parallel Approach

• Immunofluorescence Double Staining Method - Squential Approach

IHC Protocols from R&D Systems

• Fluorescent ICC Staining of Cells on Coverslips

• Fluorescent ICC Staining of Stem Cells on Coverslips

• Fluorescent ICC Staining of Non-adherent Cells

• Chromogenic IHC Staining of Paraffin-embedded Tissue Section

• Chromogenic IHC Staining of Frozen Tissue Sections

• Fluorescent IHC Staining of Paraffin-embedded Tissue Sections

• Fluorescent IHC Staining of Frozen Tissue Sections

• Heat-induced Epitope Retrieval (HIER)

• Making a 4% Formaldehyde Solution in PBS

• Gelatin-coated Slides for Histological Tissue Sections

IHC Double Staining Techniques and Protocols

• Double-label Fluorescent Immunohistochemistry with Identical Species-derived Primary Antibodies.

• Double immunofluorescent staining using two unconjugated primary antisera raised in the same species

• A simple method for immunofluorescent double staining with primary antisera from the same species

• Microwaving for double indirect immunofluorescence with primary antibodies from the same species and for staining of mouse tissues with mouse monoclonal antibodies

• A method for double immunofluorescent staining by the indirect procedure with antibodies of the same isotype

• Improved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence

• An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo- and chlorodeoxyuridine incorporated into DNA

• Double staining technique using a combination of indirect and direct immunofluorescence with monoclonal antibodies

• Immunohistochemical double staining of microwave enhanced and nonenhanced nuclear and cytoplasmic antigens

• Immunohistochemical double staining with immunogold-silver and alkaline phosphatase to identify nuclear markers of cellular proliferation

• Immunohistochemical double-staining of renal allograft tissue: critical assessment of three different protocols

• A new double labeling technique for combined in situ hybridization and immunohistochemical analysis

IHC Triple Staining Techniques and Protocols

• A triple-labeling method: HRP anterograde tract tracing combined with double immunofluorescent cell staining in developing neural tissue of the rat

• Triple Immunofluorescence Staining with Antibodies Raised in the Same Species to Study the Complex Innervation Pattern of Intrapulmonary Chemoreceptors

• Light microscopic triple-colored immunohistochemical staining on the same vibratome section using the avidin-biotin-peroxidase complex technique

• An immunoenzyme triple-staining method using both polyclonal and monoclonal antibodies from the same species. Application of combined direct, indirect, and avidin-biotin complex (ABC) technique

• Triple labeling with two-color immunofluorescence using one light source: a useful approach for the analysis of cells positive for one label and negative for the other two

• Three-color immunofluorescence histochemistry allowing triple labeling within a single section

• Triple immunological staining with colloidal gold, fluorescein and rhodamine as labels

• Rapid triple-labeling method combining in situ hybridization and double immunocytochemistry

• Triple staining of normal and degenerating nervous tissue

• Simultaneous demonstration of multiple antigens by indirect immunofluorescence or immunogold staining. Novel light and electron microscopical double and triple staining method employing primary antibodies from the same species

• Discriminative staining methods for the nervous system: luxol fast blue--periodic acid-Schiff--hematoxylin triple stain and subsidiary staining methods

       Application of IHC Double Staining Techniques and Protocols

       Application of IHC Triple Staining Techniques and Protocols