Application of IHC Double Staining Techniques and Protocols

Neuroscience Research:

• Double immunofluorescence staining of Fos and Jun in the hypothalamus of the rat

• Noncytoplasmic and filamentous appearance of calbindin-D28k and tubulin in double, indirect immunofluorescent staining of embryonic chick tissue

• The coexistence of serotonin- and substance P-like immunoreactivity in the spinal cord of the rat as shown by immunofluorescent double labeling

• Calretinin is differentially localized in magnocellular oxytocin neurons of the rat hypothalamus. A double-labeling immunofluorescence study

• Cholinergic innervation of the lateralis medialis-suprageniculate nuclear complex (LM-Sg) of the cat's thalamus: a double labeling immunohistochemical study

• Widespread immunoreactivity of presenilin in neurons of normal and Alzheimer's disease brains: double-labeling immunohistochemical study

• Variable deposition of amyloid beta-protein (A beta) with the carboxy-terminus that ends at residue valine40 (A beta 40) in the cerebral cortex of patients with Alzheimer's disease: a double-labeling immunohistochemical study with antibodies specific for A beta 40 and the A beta that ends at residues alanine42/threonine43 (A beta 42)

• Aromatic L-amino acid decarboxylase is present in serotonergic fibers of the striatum of the rat. A double-labeling immunofluorescence study

• Cholinergic and VIPergic innervation in cerebral arteries: a sequential double-labeling immunohistochemical study

• Colocalization of calbindin-D28k with vasopressin in hypothalamic cells of the rat: a double-labeling immunofluorescence study

• Double labeling immunohistochemical technique provides evidence of the specificity of glial cell markers

• Alzheimer's disease. A double-labeling immunohistochemical study of senile plaques

• L-DOPA is converted to dopamine in serotonergic fibers of the striatum of the rat: a double-labeling immunofluorescence study

• A double-labeling immunohistochemical study of tau exon 10 in Alzheimer's disease, progressive supranuclear palsy and Pick's disease

• Calcitonin and somatostatin containing C cells in rat and human thyroid. Immunohistochemical study by a double-staining method

• A new double labeling technique using tyrosine hydroxylase and dopamine-beta-hydroxylase immunohistochemistry: evidence for dopaminergic cells lying in the pons of the beef brain

• Double-labeling immunohistochemical studies on canine senile plaques and cerebral amyloid angiopathy

• Subsets of neurotensin-immunoreactive neurons in the rat striatal complex following antagonism of the dopamine D2 receptor: an immunohistochemical double-labeling study using antibodies against Fos

• Postnatal development of somatostatin- and neuropeptide Y-immunoreactive neurons in rat cerebral cortex: a double-labeling immunohistochemical study

• Heterogeneous immunoreactivity of glial cells in the mesencephalon of a lizard: a double labeling immunohistochemical study

• Coexistence of nitric oxide synthase and neuropeptides in the mouse vomeronasal organ demonstrated by a combination of double immunofluorescence labeling and a multiple dye filter

• Cell-specific localization of monocarboxylate transporters, MCT1 and MCT2, in the adult mouse brain revealed by double immunohistochemical labeling and confocal microscopy

• The spatial relationship between the perineuronal proteoglycan network and the synaptic boutons as visualized by double staining with cationic colloidal iron method and anti-calbindin-D-28K immunohistochemistry in rat cerebellar nuclei

• Immunohistochemical double-labeling study of gonadotropin-releasing hormone (GnRH)-immunoreactive cells and oxytocin-immunoreactive cells in the preoptic area of the dwarf gourami, Colisa lalia

• Demonstration of nuclear 3,5,3'-triiodothyronine receptor proteins in gonadotrophs and corticotrophs in rat anterior pituitary by double immunohistochemical staining

Cancer Research:

• Simultaneous enumeration of T-cell subsets and macrophages in bronchoalveolar lavage fluids by immunoenzyme double staining. Comparison with conventional immunofluorescence

• Application of double staining technique and incident light fluorescence microscopy to immunofluorescence studies on renal diseases

• Combined TUNEL and double immunofluorescent labeling for detection of apoptotic mononuclear phagocytes in autoimmune demyelinating disease

• TUNEL and immunofluorescence double-labeling assay for apoptotic cells with specific antigen(s)

• Biological and clinicopathological significance of endocrine differentiation of gastric adenocarcinoma evaluated by double immunohistochemical labeling for chromogranin A and bromodeoxyuridine

• Ki-67 labeling index in the differential diagnosis of exaggerated placental site, placental site trophoblastic tumor, and choriocarcinoma: a double immunohistochemical staining technique using Ki-67 and Mel-CAM antibodies

• Ki67 labelling index in gastric carcinomas. An immunohistochemical study using double staining for the evaluation of the proliferative activity of diffuse-type carcinomas

• Comparison of bromodeoxyuridine uptake and MIB 1 immunoreactivity in medulloblastomas determined with single and double immunohistochemical staining methods

• Proliferation in non-Hodgkin's lymphomas as determined by immunohistochemical double staining for Ki-67

• Immunohistochemical analysis of GH-producing adenomas--with special emphasis on plurihormonality of individual tumor cells by double staining

• Double staining immunofluorescence procedure for enumeration of cells containing cytoplasmic immunoglobulin in human bone marrow: 40 selected cases

• An immunohistochemical study using a double staining method for regional lymph nodes in gastric cancer

• Expression of blood group antigens H-2, Le(y), and sialylated-Le(a) in human colorectal carcinoma. An immunohistochemical study using double-labeling techniques

• Presence of galanin in dopaminergic neurons of the sheep infundibular nucleus: a double staining immunohistochemical study

• Periosteal osteosarcoma and parosteal chondrosarcoma evaluated by double immunohistochemical staining. Report of 2 cases

• Immunohistochemical study using a double fluorescent staining on the regional lymph nodes of gastric cancers

• Double immunohistochemical staining of HBsAg and HBcAg in hepatocellular carcinoma and its surrounding tissue

Cell Proliferation:

• Double immunohistochemical labeling technique applied to different types of cytokeratins in epithelial proliferations of the breast

• Double immunofluorescence staining of pokeweed mitogen differentiated plasma cells--a sensitive assay to ascertain purity of anti-human Ig reagents as each cell produces only one isotype

• The identity of proliferating cells in bone tumors with cartilaginous components: evaluation by double-immunohistochemical staining using proliferating cell nuclear antigen and S-100 protein

• Cell dynamics of dividing cells in the olfactory epithelium of guinea pigs, using the immunohistochemical double staining method

Others:

• Double immunofluorescent staining of alpha 2,6 sialyltransferase and beta 1,4 galactosyltransferase in monensin-treated cells: evidence for different Golgi compartments?

• Age associated changes in the distribution of lpr gene-induced B220-positive T cells in lymphoid organs of MRL/Mp-lpr/lpr mice using dual exposure microphotographs of double immunofluorescence staining

• Detection of islet cell specificity of monoclonal islet cell surface antibodies by means of double-staining immunofluorescence

• Double-immunofluorescent staining of isolated smooth muscle cells. I. preparation of anti-chicken gizzard alpha-actinin and its use with anti-chicken gizzard myosin for co-localization of alpha-actinin and myosin in chicken gizzard cells

• A one-day double-labelling technique for tissue specimens: immunogold-silver staining for in situ hybridization combined with alkaline phosphatase-anti-alkaline phosphatase (APAAP) immunohistochemistry for antigens

• Immunohistochemical double-staining for Ah receptor and ARNT in human embryonic palatal shelves

• Cell types infected in human cytomegalovirus placentitis identified by immunohistochemical double staining

• Double immunohistochemical staining for the surface antigen of lymphoid cells in situ

• Simultaneous detection of B-cells and T-cells by a double immunohistochemical technique using immunogold-silver staining and the avidin-biotin-peroxidase complex method

• Immunohistochemical double labeling of macrophages, smooth muscle cells, and apolipoprotein E in the atherosclerotic plaque