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Immunohistochemistry (IHC) Detection Systems


High Performance Polymer Detection System

High Specificity and High Sensitivity

Short Incubation Time

Competitive Price



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Immunohistochemistry, or IHC, refers to the process of detecting proteins expressed in cells of a tissue section using antigens/antibodies interaction to visualize localization of expressed proteins. Immunohistochemical staining is widely used in pathology labs and basic research to understand the distribution and localization of biomarkers in different parts of a tissue. Current IHC detection systems include Peroxisae-anti-Peroxidase (PAP), the Avidin-Biotin Complex (ABC), the Biotin-Streptavidin Amplified (B-SA) systems, and the polymer enzyme system.


IHC World is now introducing the most innovative and complete GBI IHC Detection System which includes the improved Biotin-Streptavidin amplification system SPlink and APlink, and the most powerful Polymeric amplification system Polink-1, Polink-2, and Polink-2 Plus.


SPlink (Streptavidin-Peroxidase) and APlink (Streptavidin-Alk. Phosphatase) detection kits are supplied in ready-to-use format. In this amplification system, the secondary antibody is conjugated with Biotin to its maximum extent with high binding affinity. This system not only demonstrate superior sensitivity with a super clean background, but it also provides excellent staining stability with a longer shelf life (storage period) because of an improved stabilizer formula. A shorter incubation period (10'/10' minutes for 2nd Antibody and enzyme conjugate) provides customers with faster results.


Polink-1 detection system is based on a novel polymeric technology. In this technology, the second antibody was polymeric conjugated with peroxidase or alkaline-phosphatase by using a synthetic polymer carrier. This approach increases the number of available enzymes, or ligands, binding at the antigenic site, thus increasing their reactivity to chromogen. It is a biotin-free system that eliminates non-specific binding due to endogenous biotin. It provides higher sensitivity, detection efficiency, simpler protocol, easier standardization, and less procedural variability.


Polink-2 detection system is the second generation of GBI’s polymer detection system that uses a proprietary polymer helper and polymeric HRP-linked or AP-linked secondary antibody conjugates to achieve higher sensitivity than the Polink-1 system with only one extra step. It can achieve equivalent immunostaining at a much higher dilution of primary antibodies than the first generation of polymer detection (Polink-1) system, especially on difficult-to-work antibodies. Polink-2 detection system offers laboratories the advantage of significant higher amplification of staining intensity yet lower costs resulting from more dilution of primary antibodies.


Polink-2 Plus detection system is ultra sensitive 2-step polymer detection system. The Polink-2 Plus system uses new conjugation technology and improves upon the existing 2-step polymer system through staining at 2-4 times stronger without a noticeable increase in background noise. Additional advantages of Polink-2 Plus includes: 1. Enhancement of the signal for rabbit primary antibodies including rabbit monoclonal antibodies; and 2. Detection of single species is available.


Polink DS double stain series kits use polymer-enzyme conjugates to detect two antigens simultaneously or sequentially. It gives faster and easier protocol. Polink DS system provides customers with Polymer-HRP/Polymer-AP conjugated anti-mouse, anti-rabbit, anti-rat, anti-goat with many combination options to choose from.

Polymer Detection System for Animal Tissue is especially designed to solve difficult tasks in research, such as detecting mouse antibody on mouse tissue, detecting mouse antibody on rat tissue, and detecting rat antibody on mouse tissue. It demonstrates high specificity and sensitivity.


Polymer Detection System for Human Tissue is designed to detect human antibody on human tissue  or primate tissue with clean background.

Chromogen Substrates include peroxidase substrates (DAB, AEC) and alkaline phosphatase substrates (Fast Red, AP-Red).

Mounting Mediums include aqueous, organic, permanent, anti-fade fluorescence mounting mediums for immunohistochemistry.


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