How to Build Tissue Microarrays

Using Quick-RayTM Manual Tissue Microarrayer

 

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Procedure

 

1. Place the reference slide and/or the donor block on microscope stage to position the interest tissue area and mark with an oil pen.

2. Extract (punch) the oil pen marked tissue from the donor block by using Quick-Ray® needle.

3. Deliver the extracted tissue into the intended holes of the recipient block (disposable base) pre-made by Unitma™  with Quick-Ray® needle.

4. Put the recipient block (disposable base) into the embedding mold with cutting section faced down and incubate it in Oven at 60 °C for 30 minutes.

5. Take out the recipient block (disposable base) when it turns completely transparent. Pour the paraffin (64°C) and place the cassette array on top of the paraffin.


6. Solidify the block in cold plate

7. Cutting (microtome)

 

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