How to Use the Guide to Build 1 mm Tissue Microarrays with
Quick-RayTM Manual Tissue Microarrayer
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Procedure
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1. Put the recipient block into the prop hole at bottom side. |
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2. Fit the 1mm guide on the prop. |
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3. Extract the marked tissue from the donor block by using Quick-Ray needle. |
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4. With fixing the prop by hand, insert the Quick-Ray needle into the guide hole, and put the extracted tissue into the recipient block hole by pushing the knob down. |
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5. Remove the guide and recipient block from the prop. |
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6. Put the recipient block into embedding mold with cutting section faced down and incubate it in Oven at 60 ºC for 30 minutes |
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7.
Take out the recipient block (disposable base) when it turns
completely transparent.
Pour the paraffin (64°C)
and place the cassette array on top of the paraffin.
8. Solidify the block in cold plate |
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9. Cutting (microtome) |
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In case of using 2, 3 and 5mm recipient block, insert the extracted tissue directly into the recipient block without the guide by pushing the Quick-Ray knob down. |
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