Catalog Number | Size | Price | Availability |
IW-PA1001 | 9 ml | $258 | Yes |
Description
|
ABI1 is a human homolog
of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2. ABI1
participate in the transduction of signals from Ras
to Rac by regulating Rac-specific guanine nucleotide
exchange factor (GEF) activities. ABI1 dramatically promoted
ABL1-mediated tyrosine phosphorylation of MENA, but not of
other substrates. Abi-1 regulates c-Abl-mediated
phosphorylation of Mena by interacting with both proteins.
ABI1 plays a role in the leukemogenesis by translocating to
MLL.
|
Catalog Number
|
IW-PA1001 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
|
IgG
|
Immunogen
|
A synthetic peptide
corresponding to a sequence at the C-terminal of human
Abi-1,
different from the related rat and mouse sequence by single
amino acid.
|
Purity
|
Immunogen affinity
purified
|
Conjugate
|
Unconjugated
|
Species Reactivity
|
Human,
mouse,
rat.
Not tested
in other species. |
Positive Control
|
Rat brain and spleen,
human intestine, Hela cell
|
Cellular Localization
|
Cytoplasmic
|
Form
|
Ready to use
solution. No further dilution needed. Serum blocking step
should be omitted.
|
Storage
|
Store at 2-8
°C.
Do not freeze.
|
Applications
|
IHC-P: Proteinase K
digestion is required on formalin fixed paraffin sections.
IHC-Fr: Formalin or
acetone fixed frozen section.
ICC: Acetone fixed
cells.
|
Limitations
|
This product is intended
for Research Use Only. Interpretation of the test
results is solely the responsibility of the user.
|
Precautions
|
Users should follow
general laboratory precautions when handling this product.
Wear personal protective equipment to avoid contact with
skin and eyes.
|
References
|
1.Tani,
K.; Sato, S.; Sukezane, T.; Kojima, H.; Hirose, H.; Hanafusa,
H.; Shishido, T. :
Abl interactor 1 promotes tyrosine 296 phosphorylation of
mammalian Enabled (Mena) by c-Abl kinase.
J. Biol. Chem. 278: 21685-21692, 2003.
2.
Taki, T.; Shibuya, N.; Taniwaki,
M.; Hanada, R.; Morishita, K.; Bessho, F.; Yanagisawa, M.;
Hayashi, Y. : ABI-1,
a human homolog to mouse Abl-interactor 1, fuses the MLL
gene in acute myeloid leukemia with t(10;11)(p11.2;q23).
Blood 92: 1125-1130, 1998.
|
Images
|
Immunohistochemical
analysis of paraffin-embedded sections
Rat brain tissue using
ABI-1 antibody Staining ABI-1 in cytoplasm by SABC-FITC.
Immunohistochemical
analysis of frozen sections
Rat spleen tissue using
ABI-1
antibody.
Immunohistochemical
analysis of paraffin-embedded sections
Human carcinoma of large
intestine using ABI-1 antibody.
Immunocytochemistry
analysis of Hela cell using
ABI-1 antibody.
|
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