Alpha-fetoprotein (AFP) is a major plasma protein in the fetus, where it is produced by the yolk sac and liver. Direct confirmation of the assignment of the AFP gene to chromosome 4 by in situ hybridization was provided by Harper and Dugaiczyk (1983), who placed the gene in the q11-q22 region, the same region as the albumin gene. Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA.As the major fetal serum protein, Alpha-fetoprotein is not essential for embryonic development but is required for female fertility.

IW-MA1001

9 ml

Mouse IgG2a

Human α-fetoprotein

Purified by the goat anti-mouse IgG affinity chromatography

Unconjugated

Human, mouse, rat. Not tested in other species.

Cytoplasmic

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Store at 2-8 °C. Do not freeze.

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections.

IHC-Fr: Not tested.

ICC: Not tested.

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

1. D'Eustachio, P.; Ingram, R. S.; Tilghman, S. M.; Ruddle, F. H. : Murine alpha-fetoprotein and albumin: two evolutionarily linked proteins encoded on the same mouse chromosome. Somat. Cell Genet. 7: 289-294, 1981.

2. Beattie, W. G.; Dugaiczyk, A. : Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA. Gene 20: 415-422, 1982.

3. Gabant, P.; Forrester, L.; Nichols, J.; Van Reeth, T.; De Mees, C.; Pajack, B.; Watt, A.; Smitz, J.; Alexandre, H.; Szpirer, C.; Szpirer, J. : Alpha-fetoprotein, the major fetal serum protein, is not essential for embryonic development but is required for female fertility. Proc. Nat. Acad. Sci. 99: 12865-12870, 2002.

Immunohistochemical staining of formalin fixed paraffin-embedded section of human liver cancer using AFP antibody

Immunohistochemical staining of formalin fixed paraffin-embedded human liver using AFP antibody showing cytoplasmic staining pattern of hepatocytes.

Immunohistochemical staining of formalin fixed paraffin-embedded mouse liver using AFP antibody showing cytoplasmic staining pattern of hepatocytes.

Immunohistochemical staining of formalin fixed paraffin-embedded rat liver using AFP antibody showing cytoplasmic staining pattern of hepatocytes.