Catalog Number | Size | Price | Availability |
IW-MA1001 | 9 ml | $258 | Yes |
Description
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Alpha-fetoprotein (AFP) is a major plasma protein in the fetus, where it is produced by the yolk sac and liver. Direct confirmation of the assignment of the AFP gene to chromosome 4 by in situ hybridization was provided by Harper and Dugaiczyk (1983), who placed the gene in the q11-q22 region, the same region as the albumin gene. Structure and evolution of human alpha-fetoprotein deduced from partial sequence of cloned cDNA.As the major fetal serum protein, Alpha-fetoprotein is not essential for embryonic development but is required for female fertility.
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Catalog Number
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IW-MA1001 |
Quantity
|
9 ml |
Host
|
Mouse |
Clone
|
AP-1 |
Isotype
|
Mouse
IgG2a
|
Immunogen
|
Human α-fetoprotein |
Purity
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Purified by the goat anti-mouse IgG affinity chromatography
|
Conjugate
|
Unconjugated
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Species Reactivity
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Human, mouse, rat.
Not tested in other species. |
Positive Control
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Liver |
Cellular Localization
|
Cytoplasmic
|
Form
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Ready to use solution. No further dilution needed. Serum blocking step should be omitted.
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Storage
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Store at 2-8
°C.
Do not freeze.
|
Applications
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IHC-P: Heat induced epitope retrieval is required on
formalin fixed paraffin sections.
IHC-Fr: Not tested.
ICC: Not tested.
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Limitations
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This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user.
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Precautions
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Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes.
|
References
|
1.
D'Eustachio, P.; Ingram, R. S.;
Tilghman, S. M.; Ruddle, F. H. :
Murine
alpha-fetoprotein and albumin: two evolutionarily linked
proteins encoded on the same mouse chromosome.
Somat. Cell Genet. 7: 289-294, 1981. 2.
Beattie, W. G.; Dugaiczyk, A. :
Structure and
evolution of human alpha-fetoprotein deduced from partial
sequence of cloned cDNA. Gene 20: 415-422,
1982. 3. Gabant, P.; Forrester, L.; Nichols, J.; Van Reeth, T.; De Mees, C.; Pajack, B.; Watt, A.; Smitz, J.; Alexandre, H.; Szpirer, C.; Szpirer, J. : Alpha-fetoprotein, the major fetal serum protein, is not essential for embryonic development but is required for female fertility. Proc. Nat. Acad. Sci. 99: 12865-12870, 2002. |
Images
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Immunohistochemical staining of formalin fixed paraffin-embedded section of human liver cancer using AFP antibody
Immunohistochemical staining of formalin fixed paraffin-embedded human liver using AFP antibody showing cytoplasmic staining pattern of hepatocytes.
Immunohistochemical staining of formalin fixed paraffin-embedded mouse liver using AFP antibody showing cytoplasmic staining pattern of hepatocytes.
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