Catalog Number | Size | Price | Availability |
IW-PA1005 | 9 ml | $258 | Yes |
Description
|
Angiopoietin 1 and Angiopoietin 2 are important for
development of the endothelium, by regulating tyrosine
phosphorylation of the membrane receptor Tie 2. Angiopoietin
2 is only 60% homologous with Angiopoietin 1.
Angiopoietin-2 is a naturally occurring antagonist of
angiopoietin-1 that competes for binding to the TIE2
receptor and blocks ANGPT1-induced TIE2 autophosphorylation.
Angiopoietin 1 binding to Tie 2 causes phosphorylation of
the receptor. Angiopoietin 2 competes for this binding, and
thus blocks receptor phosphorylation. Angiopoietin 2
expression occurs at sites of vascular remodelling: dorsal
aorta and major aortic branches, ovary, placenta and uterus. |
Catalog Number
|
IW-PA1005 |
Quantity
|
9 ml |
Host |
Rabbit |
Clone |
Polyclonal |
Isotype |
IgG |
Immunogen |
A synthetic peptide corresponding to a sequence mapping near
the C-terminal of human angiopoietin-2, different from the
related mouse sequence by two amino acids. |
Purity |
Immunogen affinity purified |
Conjugate |
Unconjugated |
Species Reactivity |
Human, mouse, rat.
Not tested in other species. |
Positive Control |
Placenta |
Cellular Localization |
Cytoplasmic |
Form |
Ready to use solution. No further dilution needed. Serum blocking step should be omitted. |
Storage |
Store at 2-8
°C.
Do not freeze. |
Applications |
IHC-P: Heat induced epitope retrieval is required on
formalin fixed paraffin sections.
IHC-Fr: Not tested.
ICC: Not tested. |
Limitations |
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user. |
Precautions |
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes. |
References |
1.Kim, I.; Kim, J.-H.; Ryu, Y. S.; Jung, S. H.; Nah, J. J.;
Koh, G. Y. :
Characterization and expression of a novel alternatively
spliced human angiopoietin-2. J. Biol. Chem.
275: 18550-18556, 2000.
2. Maisonpierre, P. C.; Suri, C.; Jones, P. F.; Bartunkova,
S.; Wiegand, S. J.; Radziejewski, C.; Compton, D.; McClain,
J.; Aldrich, T. H.; Papadopoulos, N.; Daly, T. J.; Davis,
S.; Sato, T. N.; Yancopoulos, G. D. :
Angiopoietin-2, a
natural antagonist for Tie2 that disrupts in vivo
angiogenesis. Science 277: 55-60, 1997.
3.Ward, E. G.; Grosios, K.; Markham, A. F.; Jones, P. F. :
Genomic structures
of the human angiopoietins show polymorphism in
angiopoietin-2. Cytogenet. Cell Genet. 94:
147-154, 2001.
|
Images |
Immunohistochemical analysis of formalin fixed paraffin-embedded
human placenta using
ANG2 antibody. |
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