Description

Annexin I, also knows as lipocortin I (Lipo1), belongs to the family of annexins. These proteins are thought to control the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes. In two lipocortins (I and II) a short amino-terminal sequence distinct from the core structure has potential regulatory functions which are  dependent on its phosphorylation state. The gene in the mouse encodes a protein of 346 amino acid residues. Mouse Lipo1 gene spans about 17 kb and is divided into 13 exons. Annexin I gene, mapped to 9q11-q22, is located on mouse chromosome 19. Annexin I acts through the formyl peptide receptor on human neutrophils. Peptides derived from the unique N-terminal domain of annexin I serve as FPR ligands and trigger different signaling pathways in a dose-dependent manner.

Catalog Number

IW-PA1006

Quantity

9 ml

Host

Rabbit

Clone

Polyclonal

Isotype

IgG

Immunogen

A synthetic peptide corresponding to a sequence near the C-terminal of human Annexin I, different from the related rat and mouse sequence by two amino acids.

Purity

Immunogen affinity purified

Conjugate

Unconjugated

Species Reactivity

Human, mouse, rat. Not tested in other species.

Positive Control

Liver, skin, cardiac muscle

Cellular Localization

Membrane, cytoplasmic or nuclear

Form

Ready to use solution. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval  (HIER) is required on formalin fixed paraffin sections. IHC-Tek Epitope Retrieval Solution (IW-1100) is recommended.

IHC-Fr: Not tested.

ICC: Suitable

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Wallner, B. P.; Mattaliano, R. J.; Hession, C.; Cate, R. L.; Tizard, R.; Sinclair, L. K.; Foeller, C.; Chow, E. P.; Browning, J. L.; Ramachandran, K. L.; Pepinsky, R. B. : Cloning and expression of human lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity. Nature 320: 77-81, 1986. .

2. Kovacic RT, Tizard R, Cate RL, Frey AZ, Wallner

BP. Correlation of gene and protein structure of rat and human lipocortin I. Biochemistry. 1991 Sep 17;30(37):9015-21.

3. Walther, A.; Riehemann, K.; Gerke, V. A novel ligand of the formyl peptide receptor: annexin I regulates neutrophil extravasation by interacting with the FPR. Molec. Cell 5: 831-840, 2000.

Images

Formalin fixed paraffin embedded human skin stained with Annexin I antibody showing cytoplasmic staining pattern.

Formalin fixed paraffin embedded mouse skin stained with Annexin I antibody showing nuclear staining pattern.