Catalog Number | Size | Price | Availability |
IW-PA1006 | 9 ml | $258 | Yes |
Description
|
Annexin I, also knows as lipocortin I (Lipo1), belongs to
the family of annexins.
These proteins are thought to control the biosynthesis of
the potent mediators of inflammation, prostaglandins and
leukotrienes. In two lipocortins (I and II) a short
amino-terminal sequence distinct from the core structure has
potential regulatory functions which are
dependent on its phosphorylation state. The gene in
the mouse encodes a protein of 346 amino acid residues.
Mouse Lipo1 gene spans about 17 kb and is divided into 13
exons. Annexin I gene, mapped to 9q11-q22, is located on
mouse chromosome 19. Annexin I acts through the formyl
peptide receptor on human neutrophils. Peptides derived from
the unique N-terminal domain of annexin I serve as FPR
ligands and trigger different signaling pathways in a
dose-dependent manner. |
Catalog Number
|
IW-PA1006 |
Quantity
|
9 ml |
Host |
Rabbit |
Clone |
Polyclonal |
Isotype |
IgG |
Immunogen |
A synthetic peptide corresponding to a sequence near the
C-terminal of human Annexin I, different from the related
rat and mouse sequence by two amino acids. |
Purity |
Immunogen affinity purified |
Conjugate |
Unconjugated |
Species Reactivity |
Human, mouse, rat.
Not tested in other species. |
Positive Control |
Liver, skin, cardiac muscle |
Cellular Localization |
Membrane, cytoplasmic or nuclear |
Form |
Ready to use solution. No further dilution needed. Serum blocking step should be omitted. |
Storage |
Store at 2-8
°C. Do not freeze. |
Applications |
IHC-P: Heat induced epitope retrieval (HIER) is required on formalin fixed
paraffin sections. IHC-Tek Epitope Retrieval Solution
(IW-1100) is recommended.
IHC-Fr: Not tested.
ICC: Suitable |
Limitations |
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user. |
Precautions |
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes. |
References |
1. Wallner, B. P.; Mattaliano, R. J.; Hession, C.; Cate, R.
L.; Tizard, R.; Sinclair, L. K.; Foeller, C.; Chow, E. P.;
Browning, J. L.; Ramachandran, K. L.; Pepinsky, R. B. :
Cloning and expression of human lipocortin, a phospholipase A2 inhibitor
with potential anti-inflammatory activity. Nature
320: 77-81, 1986. .
2.
Kovacic RT,
Tizard R,
Cate RL,
Frey AZ,
Wallner
BP. Correlation of gene and protein
structure of rat and human lipocortin I.
Biochemistry. 1991 Sep 17;30(37):9015-21.
3. Walther, A.; Riehemann, K.; Gerke, V. A novel ligand of
the formyl peptide receptor: annexin I regulates neutrophil
extravasation by interacting with the FPR.
Molec. Cell 5: 831-840, 2000. |
Images |
Formalin fixed paraffin embedded human skin stained with Annexin I antibody showing cytoplasmic staining pattern.
Formalin fixed paraffin embedded mouse skin stained with
Annexin I antibody showing nuclear staining pattern.
Immunohistochemical analysis of formalin fixed paraffin-embedded rat cardiac muscle tissue section using Annexin I antibody
|
Related Products