Catalog Number | Size | Price | Availability |
IW-PA1018 | 9 ml | $258 | Yes |
Description
|
CD22 is a surface glycoprotein of B lymphocytes that is
rapidly phosphorylated on cytoplasmic tyrosines after
antigen receptor cross-linking. CD22 is a negative regulator
of antigen receptor signaling whose onset of expression at
the mature B cell stage may serve to raise the antigen
concentration threshold required for B cell triggering. The
human CD22 gene is expressed specifically in B lymphocytes
and likely has an important function in cell-cell
interactions. The B cell coreceptor CD22 plays an important
role in regulating signal transduction via the B cell Ag
receptor.3 CD22 is located within the band region
q13.1 of chromosome 19. |
Catalog Number
|
IW-PA1018 |
Quantity
|
9 ml |
Host |
Rabbit |
Clone |
Polyclonal |
Isotype |
IgG |
Immunogen |
A peptide mapping
at the C-terminal end of human CD22, different from the
relative sequence of mouse by three amino acids. |
Purity |
Immunogen affinity purified |
Conjugate |
Unconjugated |
Species Reactivity |
Human, mouse, rat.
Not tested in other species. |
Positive Control |
Human appendicitis |
Cellular Localization |
Cytoplasmic |
Form |
Ready to use solution. No further dilution needed. Serum blocking step should be omitted. |
Storage |
Store at 2-8
°C. Do not freeze. |
Applications |
IHC-P: Heat induced epitope retrieval enhance stain on
formalin fixed paraffin sections.
IHC-Fr: Formalin or acetone fixed tissues.
ICC: Suitable. |
Limitations |
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user. |
Precautions |
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes. |
References |
1.
O'Keefe, T. L.; Williams, G. T.; Davies, S. L.;
Neuberger, M. S.
Hyperresponsive B cells in CD22-deficient mice.
Science 274: 798-801, 1996.
2.
Wilson, G. L.; Najfeld, V.; Kozlow, E.;
Menniger, J.; Ward, D.; Kehrl, J. H.
Genomic structure
and chromosomal mapping of the human CD22 gene. J.
Immun. 150: 5013-5024, 1993.
John, B.; Herrin, B. R.; Raman, C.; Wang, Y.;
Bobbitt, K. R.; Brody, B. A.; Justement, L. B.
The B cell coreceptor CD22 associates with AP50, a clathrin-coated pit
adapter protein, via tyrosine-dependent interaction.
J. Immun. 170: 3534-3543, 2003. |
Images |
Immunohistochemical analysis of formalin fixed paraffin-embedded
human appendicitis using CD22 antibody |
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