Description

The alpha-beta heterodimeric T-cell antigen receptor binds antigen in association with major histocompatibility complex proteins on host cell surfaces. These 2 disulfide-linked glycoproteins, TCRA and TCRB are associated on T-cell surfaces with a complex of proteins called CD3 (formerly T3). Human CD3 consists of at least 4 proteins: gamma, delta , epsilon, and zeta . The CD3-gamma/CD3-delta gene pair is within 300 kb of the CD3-epsilon gene and therefore these genes form a tightly linked cluster on 11q23. The clustering may be significant in terms of their simultaneous activations during T-cell development.

Catalog Number

IW-MA1015

Quantity

9 ml

Host

Mouse

Clone

CA-3

Isotype

Mouse IgG1

Immunogen

Human thymocytes followed by Sezary T cells.

Purity

Purified by the goat anti-mouse IgG affinity chromatography.

Conjugate

Unconjuated

Species Reactivity

Human, rat. Not tested in other species.

Positive Control

Human tonsil

Cellular Localization

Membrane

Form

Ready to use solution in PBS based antibody dilution buffer containing 0.01% sodium azide. No further dilution needed. Serum blocking step should be omitted.

Storage

Store at 2-8 °C. Do not freeze.

Applications

IHC-P: Heat induced epitope retrieval is required on formalin fixed paraffin sections. IHC-Tek Epitope Retrieval Solution (IW-1100) is recommended.

IHC-Fr: Formalin fixed or acetone fixed frozen sections.

ICC: Not tested.

Limitations

This product is intended for Research Use Only. Interpretation of the test results is solely the responsibility of the user.

Precautions

Users should follow general laboratory precautions when handling this product. Wear personal protective equipment to avoid contact with skin and eyes.

References

1. Krissansen, G. W.; Owen, M. J.; Verbi, W.; Crumpton, M. J. : Primary structure of the T3 gamma subunit of the T3/T cell antigen receptor complex deduced from cDNA sequences: evolution of the T3 gamma and delta subunits. EMBO J. 5: 1799-1808, 1986.

2. Tunnacliffe, A.; Buluwela, L.; Rabbitts, T. H. : Physical linkage of three CD3 genes on human chromosome 11. EMBO J. 6: 2953-2957, 1987.

Images

Immunohistochemical staining of formalin fixed paraffin-embedded human tonsil using CD3 antibody. DAB chromogen substrate.