Catalog Number | Size | Price | Availability |
IW-PA1021 | 9 ml | $258 | Yes |
Description
|
The CD44 gene, which is a transmembrane protein, is
expressed as a family of molecular isoforms generated from
alternative RNA splicing and posttranslational
modifications. The gene, which contains 19 exons spanning
some 50 kb of genomic DNA, is a
widely expressed integral membrane protein that acts as a
receptor for hyaluronan (HA) and is important to
cell-extracellular matrix interaction. CD44 binding
with HA can play an important role in cellular aggregation
and tumor cell growth. CD44 is
necessary for limb development and functions in a novel
growth factor presentation mechanism. A specific CD44
splice variant is crucial for the proliferation of these
mesenchymal cells. CD44
glycoproteins are involved in leukocyte extravasation but
also in the regulation of growth factor activation,
stability, and signaling. Moreover, it plays a
pivotal role in arteriogenesis.
|
Catalog Number
|
IW-PA1021 |
Quantity
|
9 ml |
Host
|
Rabbit
|
Clone
|
Polyclonal
|
Isotype
|
IgG
|
Immunogen
|
A peptide mapping at the C-terminal end of human CD44,
identical to the related rat and mouse sequence.
|
Purity
|
Immunogen affinity purified
|
Conjugate
|
Unconjugated
|
Species Reactivity
|
Human, mouse, rat.
Not tested in other species. |
Positive Control
|
Human tonsil, thymus, spleen, skin.
|
Cellular Localization
|
Membrane |
Form
|
Ready to use solution. No further dilution needed. Serum
blocking step should be omitted.
|
Storage
|
Store at 2-8
°C.
Do not freeze.
|
Applications
|
IHC-P: Heated Induced Epitope Retrieval or Proteinase K digestion is required on formalin fixed
paraffin sections.
IHC-Fr: Acetone fixed frozen sections. ICC: Acetone fixed cultured cells.
|
Limitations
|
This product is intended for Research Use Only.
Interpretation of the test results is solely the
responsibility of the user.
|
Precautions
|
Users should follow general laboratory precautions when
handling this product. Wear personal protective equipment to
avoid contact with skin and eyes.
|
References
|
1.
Screaton GR,
Bell MV,
Jackson DG,
Cornelis FB,
Gerth U,
Bell JI.
Genomic structure of DNA encoding the lymphocyte homing
receptor CD44 reveals at least 12 alternatively spliced
exons.
Proc Natl Acad Sci U S A. 1992 Dec 15;
89(24):12160-4.
4.
Sherman L,
Wainwright D,
Ponta H,
Herrlich P.
A splice variant of
CD44 expressed in the apical ectodermal ridge presents
fibroblast growth factors to limb mesenchyme and is required
for limb outgrowth.
Genes Dev. 1998 Apr 1; 12(7):1058-71.
|
Images
|
Immunohistochemical analysis of formalin fixed
paraffin-embedded human mammary cancer using CD44 antibody
|
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